the next generation of scientists in order to tackle the challenges of diabetic bone disease
from various angles and with the newest technologies available. Interdisciplinary training and
implementation of innovative approaches are key. Within this consortium, we will
comprehensively unravel the genetic and environmental mechanisms that contribute to bone
fragility in diabetes, identify predictors and clinical markers for patient stratification, decipher
the underlying molecular mechanisms of bone fragility in diabetes, and establish potential
interventions through a personalised medicine approach.
The research programme will address different aspects of diabetic bone disease from the
viewpoints of epidemiology, genetics, miRNAs, microbiome, bone biology, bone
biomechanics and microstructure, preclinical and clinical research. It will utilise advanced
imaging and computational approaches, diabetes mouse models and access to clinical
cohorts and registry data to obtain a comprehensive overview of how these mechanisms
combine in diabetes to cause increased fracture risk.
With this interdisciplinary approach, we can explore the impact of biological pathways in
mouse models and/or humans, and interactions with diet, exercise and other exposures.
Collaborations with industry will allow early identification of IP, access to state of the art
technologies, and will complement the academic ESR training programme with
entrepreneurship and industrial mentoring.
Your PhD project:
One Phd project will examine the cellular and molecular phenotype of human skeletal (MSC)
and osteoblastic cultures established from bone marrow aspirates obtained from patients
with T1D/T2D and healthy, age-matched controls and include ex vivo cell proliferation,
osteoblastic and adipocytic differentiation, molecular phenotyping using RNA-seq. Ex vivo
metabolic studies of bioenergetics will be included e.g. measuring glycolysis and OXPHOS.
The role of miRNA species in regulating energy metabolism will be determined using either
global miRNA gene expression analysis or focused analysis of miRNAs.
One Phd project is based on epidemiological analysis of the effect of metformin in bone
outcomes and fracture. Metformin use in relation to fracture risk will be examined in the
registry of Statistics Denmark and compared with the findings in the Rotterdam Study.
You will embark on secondments to other FIDELIO partners TamiRNA (AT) or ERASMUS to
access experimental models or tools or receive training not available in the home laboratory.
Total secondment time is 3 months.